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AHA-1 antibody [N1C1]

Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312
Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312
Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312
Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312
Anti-AHA-1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102312

Cat No. GTX102312

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AHA-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

activator of HSP90 ATPase activity 1 , AHA1 , C14orf3 , hAha1 , p38

Cellular Localization

Cytoplasm , cytosol , Endoplasmic reticulum

Background

Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.

Database

DATA IMAGES
Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312

GTX102312 WB Image

Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312

GTX102312 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
10% SDS PAGE
GTX102312 diluted at 1:1000

Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312

GTX102312 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using AHA-1 (GTX102312) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312

GTX102312 IHC-P Image

AHA-1 antibody [N1C1] detects AHA-1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AHA-1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102312

GTX102312 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using AHA-1(GTX102312) antibody at 1:500 dilution.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169