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AHA-1 antibody [N1C1]

KO/KD
Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312
Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312
Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312
Anti-AHA-1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102312
Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312

Cat. No. GTX102312

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AHA-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AHA-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949608

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activator of HSP90 ATPase activity 1 , AHA1 , C14orf3 , hAha1 , p38

Cellular Localization

Cytoplasm , cytosol , Endoplasmic reticulum

Background

Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.

Database

Research Area

DATA IMAGES

Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312

GTX102312 WB Image

KO/KD

Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AHA-1 antibody [N1C1] used in Western Blot (WB). GTX102312

GTX102312 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
10% SDS PAGE
GTX102312 diluted at 1:1000

Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312

GTX102312 IHC-P Image

AHA-1 antibody [N1C1] detects AHA-1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AHA-1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102312

GTX102312 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using AHA-1(GTX102312) antibody at 1:500 dilution.

Anti-AHA-1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102312

GTX102312 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using AHA-1 (GTX102312) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kim D et al. Sci Rep 2021; 11 (1) : 19946 AHA1 regulates cell migration and invasion via the EMT pathway in colorectal adenocarcinomas.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169