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AHA-1 antibody [N2C3]

KO/KD
Anti-AHA-1 antibody [N2C3] used in Western Blot (WB). GTX102321
Anti-AHA-1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX102321
Anti-AHA-1 antibody [N2C3] used in Western Blot (WB). GTX102321
Anti-AHA-1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102321
Anti-AHA-1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102321

Cat. No. GTX102321

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all AHA-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:30000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

JurKat , HeLa

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AHA-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240431

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activator of HSP90 ATPase activity 1 , AHA1 , C14orf3 , hAha1 , p38

Cellular Localization

Cytoplasm , cytosol , Endoplasmic reticulum

Background

Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.

Database

Research Area

DATA IMAGES

Anti-AHA-1 antibody [N2C3] used in Western Blot (WB). GTX102321

GTX102321 WB Image

KO/KD

Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N2C3] (GTX102321) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AHA-1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX102321

GTX102321 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using AHA-1(GTX102321) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AHA-1 antibody [N2C3] used in Western Blot (WB). GTX102321

GTX102321 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
10% SDS PAGE
AHA-1 antibody
GTX102321 diluted at 1:1000

Anti-AHA-1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102321

GTX102321 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using AHA-1(GTX102321) antibody at 1:500 dilution.

Anti-AHA-1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102321

GTX102321 ICC/IF Image

AHA-1 antibody [N2C3] detects AHA-1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AHA-1 stained by AHA-1 antibody [N2C3] (GTX102321) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169