Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N2C3] (GTX102321) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AHA-1 antibody [N2C3] detects AHA-1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AHA-1 stained by AHA-1 antibody [N2C3] (GTX102321) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Immunofluorescence analysis of methanol-fixed A431, using AHA-1(GTX102321) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: JurKat
10% SDS PAGE
AHA-1 antibody
GTX102321 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using AHA-1(GTX102321) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min