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AHCY antibody [N3C3]

Anti-AHCY antibody [N3C3] used in Western Blot (WB). GTX101387
Anti-AHCY antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101387
Anti-AHCY antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101387

Cat. No. GTX101387

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AHCY products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Drosophila, Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

8.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AHCY. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240430

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosylhomocysteinase , SAHH , adoHcyase

Cellular Localization

Cytoplasm , Melanosome

Background

S-adenosylhomocysteine hydrolase catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AHCY antibody [N3C3] used in Western Blot (WB). GTX101387

GTX101387 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101387 diluted at 1:1000

Anti-AHCY antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101387

GTX101387 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using AdoHcyase(GTX101387) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AHCY antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101387

GTX101387 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using AdoHcyase(GTX101387) antibody at 1:500 dilution.

REFERENCE

Application Reference

Cassiano C et al. Front Chem 2019; 7 : 53 Chemical Proteomics-Guided Identification of a Novel Biological Target of the Bioactive Neolignan Magnolol.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169