GeneTex
United States (US)

AHCY antibody [N3C3]

GTX101387_WB_19011506_724.jpg
GTX101387_39715_IFA_19011506_152.jpg
GTX101387_39715_IHC_19011506_378.jpg

Cat No. GTX101387

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

8.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AHCY. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adenosylhomocysteinase,Sahh,Adohcyase,Ahcy

Cellular Localization

Cytoplasm , Melanosome

Background

S-adenosylhomocysteine hydrolase catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101387_WB_19011506_724.jpg

GTX101387 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101387 diluted at 1:1000

GTX101387_39715_IFA_19011506_152.jpg

GTX101387 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using AdoHcyase(GTX101387) antibody at 1:500 dilution.

GTX101387_39715_IHC_19011506_378.jpg

GTX101387 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using AdoHcyase(GTX101387) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf