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AHR antibody [RPT1]

Anti-AHR antibody [RPT1] used in IHC (Paraffin sections) (IHC-P). GTX22770
Anti-AHR antibody [RPT1] used in Western Blot (WB). GTX22770
Anti-AHR antibody [RPT1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22770

Cat No. GTX22770

Host

Mouse

Clonality

Monoclonal

Clone Name

RPT1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay

Reactivity

Human, Mouse, Rat, Pig, Primate
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:100-1:1000
IHC-P 1:10 - 1:100
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384840

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aryl hydrocarbon receptor , bHLHe76

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-AHR antibody [RPT1] used in IHC (Paraffin sections) (IHC-P). GTX22770

GTX22770 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-AHR antibody [RPT1] used in Western Blot (WB). GTX22770

GTX22770 WB Image

WB analysis of 20 ug of HepG2, U2OS, and K562 whole cell lysates using GTX22770 AHR antibody [RPT1].
Dilution : 1:1000

Anti-AHR antibody [RPT1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22770

GTX22770 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX22770 AHR antibody [RPT1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339