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AHR antibody [RPT1]

Anti-AHR antibody [RPT1] used in IHC (Paraffin sections) (IHC-P). GTX22770
Anti-AHR antibody [RPT1] used in Western Blot (WB). GTX22770
Anti-AHR antibody [RPT1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22770

Cat. No. GTX22770

Reference 10

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RPT1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay

Reactivity

 Human, Mouse, Rat, Pig, Primate
Package
100 μl ($399)
See all AHR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:100-1:1000
IHC-P 1:10 - 1:100
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384840

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor , bHLHe76

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-AHR antibody [RPT1] used in IHC (Paraffin sections) (IHC-P). GTX22770

GTX22770 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-AHR antibody [RPT1] used in Western Blot (WB). GTX22770

GTX22770 WB Image

WB analysis of 20 ug of HepG2, U2OS, and K562 whole cell lysates using GTX22770 AHR antibody [RPT1].
Dilution : 1:1000

Anti-AHR antibody [RPT1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22770

GTX22770 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX22770 AHR antibody [RPT1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

REFERENCE

Application Reference

H Shang et al. Cell Biosci 2023; 13 (1) : 127 Gut microbiota-derived tryptophan metabolites alleviate liver injury via AhR/Nrf2 activation in pyrrolizidine alkaloids-induced sinusoidal obstruction syndrome
Application : WB
Zhong C et al. Am J Cancer Res 2022; 12 (6) : 2558-2575 TDO2 and tryptophan metabolites promote kynurenine/AhR signals to facilitate glioma progression and immunosuppression.
Iizuka T et al. Proc Natl Acad Sci U S A 2022; 119 (47) : e2208886119 Mono-(2-ethyl-5-hydroxyhexyl) phthalate promotes uterine leiomyoma cell survival through tryptophan-kynurenine-AHR pathway activation.
Application : ICC/IF
Reactivity : Human
Zhan Y et al. BMC Med 2022; 20 (1) : 365 NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma.
Xiaoyuan Ma et al. Front Microbiol 2022; 13 : 802409 The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis.
Reactivity : Mouse
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REVIEW

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Application Reference
H Shang et al. Cell Biosci 2023; 13 (1):127 Gut microbiota-derived tryptophan metabolites alleviate liver injury via AhR/Nrf2 activation in pyrrolizidine alkaloids-induced sinusoidal obstruction syndrome
Application : WB
Zhong C et al. Am J Cancer Res 2022; 12 (6):2558-2575 TDO2 and tryptophan metabolites promote kynurenine/AhR signals to facilitate glioma progression and immunosuppression.
Iizuka T et al. Proc Natl Acad Sci U S A 2022; 119 (47):e2208886119 Mono-(2-ethyl-5-hydroxyhexyl) phthalate promotes uterine leiomyoma cell survival through tryptophan-kynurenine-AHR pathway activation.
Application : ICC/IF
Reactivity : Human
Zhan Y et al. BMC Med 2022; 20 (1):365 NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma.
Xiaoyuan Ma et al. Front Microbiol 2022; 13:802409 The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis.
Reactivity : Mouse
Reynolds WJ et al. FASEB J 2020; 34 (11):14725-14735 Exposing human primary dermal fibroblasts to particulate matter induces changes associated with skin aging.
Application : ICC/IF
Reactivity : Human
Sadik A et al. Cell 2020; 182 (5):1252-1270.e34 IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression.
Application : WB
Reactivity : Human
Su HH et al. Toxicology 2016; 370:116-126 Aryl hydrocarbon receptor-ligand axis mediates pulmonary fibroblast migration and differentiation through increased arachidonic acid metabolism.
Application : ChIP assay
Reactivity : Human
Mariuzzi L et al. Lab Invest 2016; (Epub) Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.
Application : IHC-P
Reactivity : Human
Floreani M et al. PLoS One 2013; 8 (4):e61983 Severe liver cirrhosis markedly reduces AhR-mediated induction of cytochrome P450 in rats by decreasing the transcription of target genes.
Application : WB
Reactivity : Rat
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399