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AHR antibody [RPT9]

Anti-AHR antibody [RPT9] used in Flow cytometry (FACS). GTX22769
Anti-AHR antibody [RPT9] used in (). GTX22769
Anti-AHR antibody [RPT9] used in Flow cytometry (FACS). GTX22769
Anti-AHR antibody [RPT9] used in Flow cytometry (FACS). GTX22769
Anti-AHR antibody [RPT9] used in (). GTX22769
Anti-AHR antibody [RPT9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22769
Anti-AHR antibody [RPT9] used in Western Blot (WB). GTX22769

Cat. No. GTX22769

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RPT9

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all AHR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10-1:100
IHC-Fr Assay dependent
FACS 1:50
IP Assay dependent
ELISA Assay dependent
ChIP assay 1:100
Gel supershift assays Assay dependent
IHC Assay dependent

Note :

ICC/IF
Permeabilization : 0.2% Triton X-100 in PBS for 30 min at RT.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

buffer

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor

Background

binds 3-methylcholanthrene and other aromatic hydrocarbons; may play a role in bone formation [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-AHR antibody [RPT9] used in Flow cytometry (FACS). GTX22769

GTX22769 FACS Image

FACS analysis of NIH/3T3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue).
Dilution : 1:50 for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-AHR antibody [RPT9] used in (). GTX22769

GTX22769 Image

ICC/IF analysis of U251 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-AHR antibody [RPT9] used in Flow cytometry (FACS). GTX22769

GTX22769 FACS Image

FACS analysis of HeLa cells using GTX22769 AHR antibody
Dilution : 1:50 for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature[RPT9] compared to an isotype control (blue).

Anti-AHR antibody [RPT9] used in Flow cytometry (FACS). GTX22769

GTX22769 FACS Image

FACS analysis of PC-3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue).
Dilution : 1:50 for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-AHR antibody [RPT9] used in (). GTX22769

GTX22769 Image

ICC/IF analysis of HeLa Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-AHR antibody [RPT9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22769

GTX22769 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-AHR antibody [RPT9] used in Western Blot (WB). GTX22769

GTX22769 WB Image

WB analysis of 20 μg of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (Lane B) or empty vector control (Lane A) using GTX22769 AHR antibody [RPT9].
Dilution : 1:1000

REFERENCE

Application Reference

Zhou N et al. J Clin Invest 2023; 133 (24) Aryl hydrocarbon receptor sulfenylation promotes glycogenolysis and rescues cancer chemoresistance.
Tseng YH et al. Ecotoxicol Environ Saf 2021; 210 : 111892 Benzo[a]pyrene induces fibrotic changes and impairs differentiation in lung stem cells.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399