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AHR antibody

Anti-AHR antibody used in Western Blot (WB). GTX129013
Anti-AHR antibody used in Western Blot (WB). GTX129013
Anti-AHR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129013

Cat No. GTX129013

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
See all AHR products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AHR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aryl Hydrocarbon Receptor,Bhlhe76,Ahr

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-AHR antibody used in Western Blot (WB). GTX129013

GTX129013 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AHR antibody (GTX129013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-AHR antibody used in Western Blot (WB). GTX129013

GTX129013 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AHR antibody (GTX129013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-AHR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129013

GTX129013 ICC/IF Image

AHR antibody detects AHR protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AHR stained by AHR antibody (GTX129013) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.
REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169