GeneTex
United States (US)

AICDA antibody

GTX127276_41010_WB_18121410_120.jpg
GTX127276_41010_IFA_18121410_549.jpg
GTX127276_41010_IHC_18121410_360.jpg

Cat No. GTX127276

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AICDA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Activation Induced Cytidine Deaminase,Aid,Arp2,Cda2,Hel-S-284,Higm2,Aicda

Background

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq]

Database

DATA IMAGES
GTX127276_41010_WB_18121410_120.jpg

GTX127276 WB Image

Sample (30 µg of whole cell lysate)
A: K562
B: THP-1
C: HL-60
12% SDS PAGE
GTX127276 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127276_41010_IFA_18121410_549.jpg

GTX127276 ICC/IF Image

AICDA antibody detects AICDA protein at cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AICDA protein stained by AICDA antibody (GTX127276) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX127276_41010_IHC_18121410_360.jpg

GTX127276 IHC-P Image

AICDA antibody detects AICDA protein at cytosol on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
AICDA antibody (GTX127276) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169