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AIF antibody [AT22E9]

Anti-AIF antibody [AT22E9] used in Western Blot (WB). GTX57664
Anti-AIF antibody [AT22E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57664
Anti-AIF antibody [AT22E9] used in Western Blot (WB). GTX57664

Cat. No. GTX57664

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT22E9

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all AIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT22E9 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AIFM3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apoptosis inducing factor mitochondria associated 1 , AIF , AIFM1 , CMT2D , CMTX4 , COWCK , COXPD6 , DFNX5 , NADMR , NAMSD , PDCD8 , apoptosis inducing factor, mitochondria associated 1

Cellular Localization

Mitochondrion intermembrane space,Mitochondrion inner membrane,Cytoplasm,Nucleus,Perinuclear region

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-AIF antibody [AT22E9] used in Western Blot (WB). GTX57664

GTX57664 WB Image

WB analysis of various samples using GTX57664 AIF antibody.
Lane 1 : Jurkat whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Hep3B whole cell lysate
Lane 4 : Raji whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : MCF-7 whole cell lysate
Lane 7 : CTLL2 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-AIF antibody [AT22E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57664

GTX57664 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57664 AIF antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-AIF antibody [AT22E9] used in Western Blot (WB). GTX57664

GTX57664 WB Image

WB analysis of various samples using GTX57664 AIF antibody.
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399