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AIF antibody [N1C1]

KO/KD
Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306
Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306
Anti-AIF antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113306
Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306
Anti-AIF antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113306
Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306

Cat. No. GTX113306

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all AIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Mouse liver , H1299 , HepG2 , rat liver , 293T

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036190

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apoptosis inducing factor mitochondria associated 1 , AIF , CMT2D , CMTX4 , COWCK , COXPD6 , DFNX5 , NADMR , NAMSD , PDCD8

Cellular Localization

Mitochondrion intermembrane space , Nucleus

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306

GTX113306 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.

Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306

GTX113306 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX113306 diluted at 1:1000

Anti-AIF antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113306

GTX113306 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using AIF(GTX113306) antibody at 1:200 dilution.

Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306

GTX113306 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX113306 diluted at 1:1000

Anti-AIF antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113306

GTX113306 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using AIF(GTX113306) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AIF antibody [N1C1] used in Western Blot (WB). GTX113306

GTX113306 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.

REFERENCE

Application Reference

Ma PW et al. Oxid Med Cell Longev 2022; 2022 : 3373828 Treatment with the Ferroptosis Inhibitor Ferrostatin-1 Attenuates Noise-Induced Hearing Loss by Suppressing Ferroptosis and Apoptosis.
Geng Q et al. Exp Gerontol 2021; 151 : 111429 Association between Cav3 channel upregulation in spiral ganglion neurons and age-dependent hearing loss.
Shimada M et al. Sci Rep 2020; 10 (1) : 14195 The human papillomavirus E6 protein targets apoptosis-inducing factor (AIF) for degradation.
Application : WB
Reactivity : Human
Murata MM et al. Mol Biol Cell 2019; 30 (20) : 2584-2597 NAD+ consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival.
Shen H et al. Front Aging Neurosci 2018; 10 : 353 Age-Dependent Up-Regulation of HCN Channels in Spiral Ganglion Neurons Coincide With Hearing Loss in Mice.
Application : ICC/IF
Reactivity : Mouse
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REVIEW

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Application Reference
Ma PW et al. Oxid Med Cell Longev 2022; 2022:3373828 Treatment with the Ferroptosis Inhibitor Ferrostatin-1 Attenuates Noise-Induced Hearing Loss by Suppressing Ferroptosis and Apoptosis.
Geng Q et al. Exp Gerontol 2021; 151:111429 Association between Cav3 channel upregulation in spiral ganglion neurons and age-dependent hearing loss.
Shimada M et al. Sci Rep 2020; 10 (1):14195 The human papillomavirus E6 protein targets apoptosis-inducing factor (AIF) for degradation.
Application : WB
Reactivity : Human
Murata MM et al. Mol Biol Cell 2019; 30 (20):2584-2597 NAD+ consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival.
Shen H et al. Front Aging Neurosci 2018; 10:353 Age-Dependent Up-Regulation of HCN Channels in Spiral Ganglion Neurons Coincide With Hearing Loss in Mice.
Application : ICC/IF
Reactivity : Mouse
Lin SC et al. Oncol Rep 2018; 39 (2):703-710 Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.
Application : WB
Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Application : WB, ICC/IF
Reactivity : Human
Kuo HF et al. PLoS One 2016; 11 (5):e0156059 Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.
Application : WB
Reactivity : Human
Ma C et al. Life Sci 2016; 144:113-20 Pre-administration of BAX-inhibiting peptides decrease the loss of the nigral dopaminergic neurons in rats.
Application : IHC-P
Reactivity : Rat
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169