Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using AIF(GTX113306) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX113306 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using AIF(GTX113306) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX113306 diluted at 1:1000