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AIF antibody [N1N2], N-term

KO/KD
Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399
Anti-AIF antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102399
Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399
Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399
Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399

Cat. No. GTX102399

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human AIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949609

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apoptosis inducing factor mitochondria associated 1 , AIF , CMT2D , CMTX4 , COWCK , COXPD6 , DFNX5 , NADMR , NAMSD , PDCD8

Cellular Localization

Mitochondrion intermembrane space , Nucleus

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399

GTX102399 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term (GTX102399) diluted at 1:4000.

Anti-AIF antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102399

GTX102399 ICC/IF Image

AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399

GTX102399 WB Image

AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
AIF antibody [N1N2], N-term (GTX102399) dilution: 1:1000

Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399

GTX102399 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AIF(GTX102399) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399

GTX102399 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using AIF(GTX102399) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Adriaenssens E et al. Nat Cell Biol 2023; 25 (3) : 467-480 Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169