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AIF antibody [N1N2], N-term

Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399
Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399
Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399
Anti-AIF antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102399
Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399

Cat No. GTX102399

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human AIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

apoptosis inducing factor mitochondria associated 1 , AIF , CMT2D , CMTX4 , COWCK , COXPD6 , DFNX5 , NADMR , NAMSD , PDCD8

Cellular Localization

Mitochondrion intermembrane space , Nucleus

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399

GTX102399 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term (GTX102399) diluted at 1:4000.

Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399

GTX102399 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AIF(GTX102399) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AIF antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102399

GTX102399 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using AIF(GTX102399) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AIF antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102399

GTX102399 ICC/IF Image

AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-AIF antibody [N1N2], N-term used in Western Blot (WB). GTX102399

GTX102399 WB Image

AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
7.5 % SDS-PAGE
AIF antibody [N1N2], N-term (GTX102399) dilution: 1:1000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169