GeneTex
United States (US)

AIF antibody

KO/KD
GTX32428_20180530_IHC-P_18121410_238.jpg
GTX32428_20180530_WB_18121410_995.jpg
GTX32428_20180822_WB_18121410_988.jpg
GTX32428_20180530_IHC-P_2_18121410_302.jpg
GTX32428_20180530_IHC-P_3_18121410_739.jpg
GTX32428_20180530_IHC-P_1_18121410_978.jpg
GTX32428_20180530_ICCIF_18121410_365.jpg

Cat No. GTX32428

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

67 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIFM1 (NP_004199.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Apoptosis Inducing Factor Mitochondria Associated 1,Aif,Cmt2D,Cmtx4,Cowck,Coxpd6,Dfnx5,Nadmr,Namsd,Pdcd8,Aifm1

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES
GTX32428_20180530_IHC-P_18121410_238.jpg

GTX32428 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX32428 AIF antibody.
Dilution : 1:200

GTX32428_20180530_WB_18121410_995.jpg

GTX32428 WB Image

WB analysis of various samples using GTX32428 AIF antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32428_20180822_WB_18121410_988.jpg

GTX32428 WB Image

KO/KD

WB analysis of wild type and AIF knockout cell lysate using GTX32428 AIF antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32428_20180530_IHC-P_2_18121410_302.jpg

GTX32428 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32428 AIF antibody.
Dilution : 1:200

GTX32428_20180530_IHC-P_3_18121410_739.jpg

GTX32428 IHC-P Image

IHC-P analysis of mouse esophagus tissue using GTX32428 AIF antibody.
Dilution : 1:200

GTX32428_20180530_IHC-P_1_18121410_978.jpg

GTX32428 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32428 AIF antibody.
Dilution : 1:100

GTX32428_20180530_ICCIF_18121410_365.jpg

GTX32428 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32428 AIF antibody.
Blue : DAPI

Package List Price ($)
$ 339