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AIF antibody

Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428
Anti-AIF antibody used in Western Blot (WB). GTX32428
Anti-AIF antibody used in Western Blot (WB). GTX32428
Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428
Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428
Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428
Anti-AIF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32428

Cat No. GTX32428

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIFM1 (NP_004199.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apoptosis inducing factor mitochondria associated 1 , AIF , CMT2D , CMTX4 , COWCK , COXPD6 , DFNX5 , NADMR , NAMSD , PDCD8

Cellular Localization

Mitochondrion inner membrane,Cytoplasm,Nucleus

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428

GTX32428 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX32428 AIF antibody.
Dilution : 1:200

Anti-AIF antibody used in Western Blot (WB). GTX32428

GTX32428 WB Image

WB analysis of various samples using GTX32428 AIF antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-AIF antibody used in Western Blot (WB). GTX32428

GTX32428 WB Image

WB analysis of wild type and AIF knockout cell lysate using GTX32428 AIF antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428

GTX32428 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32428 AIF antibody.
Dilution : 1:200

Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428

GTX32428 IHC-P Image

IHC-P analysis of mouse esophagus tissue using GTX32428 AIF antibody.
Dilution : 1:200

Anti-AIF antibody used in IHC (Paraffin sections) (IHC-P). GTX32428

GTX32428 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32428 AIF antibody.
Dilution : 1:100

Anti-AIF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32428

GTX32428 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32428 AIF antibody.
Blue : DAPI

REFERENCE

There are currently no references for AIF antibody (GTX32428). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 339