AIMP1 antibody [AT6D10]

Cat. No. GTX57649

Host	Mouse
Clonality	Monoclonal
Clone Name	AT6D10
Isotype	IgG2a
Application	WB, ICC/IF, FACS
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX57649 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT6D10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AIMP3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aminoacyl tRNA synthetase complex interacting multifunctional protein 1 , AIMP1 , EMAP2 , EMAPII , HLD3 , SCYE1 , p43 , EMAP II

Cellular Localization

Nucleus,Cytoplasm,Cytosol,Cytoplasmic vesicle,Secretory vesicle,Secreted,Endoplasmic reticulum,Golgi apparatus

Background

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

Database

Gene ID: 9255 AIMP1
UniProt: Q12904 AIMP1

Research Area

DATA IMAGES

GTX57649 WB Image

WB analysis of various samples using GTX57649 AIMP1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Raji whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A549 whole cell lysate
Lane 6 : HaCaT whole cell lysate
Loading : 40 μg
Dilution : 1:1000

GTX57649 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57649 AIMP1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57649 FACS Image

FACS analysis of Hep3B cells using GTX57649 AIMP1 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty