APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , HepG2
Predict Reactivity
Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human AIMP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951828
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aminoacyl tRNA synthetase complex interacting multifunctional protein 1 , EMAP2 , EMAPII , HLD3 , SCYE1 , p43
Cellular Localization
Nucleus , Cytoplasm , cytosol , Cytoplasmic vesicle , secretory vesicle , Secreted , Endoplasmic reticulum , Golgi apparatus
Background
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105175 WB Image
Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
12% SDS PAGE
GTX105175 diluted at 1:1000
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GTX105175 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1(GTX105175) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX105175 IHC-P Image
AIMP1 antibody detects SCYE1 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
AIMP1 antibody (GTX105175) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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