GeneTex
United States (US)

AIMP1 antibody

GTX105175_39827_IFA_19011506_519.jpg
GTX105175_39827_IHC_19011506_873.jpg
GTX105175_WB_19011506_725.jpg

Cat No. GTX105175

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

H1299 , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AIMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EMAP2 antibody, EMAPII antibody, SCYE1 antibody, p43 antibody, AIMP1 antibody, small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody, endothelial-monocyte activating polypeptide II antibody, aminoacyl tRNA synthase complex-interacting multifunctional protein 1 antibody, endothelial monocyte-activating polypeptide 2 antibody, ARS-interacting multifunctional protein 1 antibody, multisynthetase complex auxiliary component p43 antibody, multisynthase complex auxiliary component p43 antibody, aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody

Cellular Localization

Nucleus , Cytoplasm , cytosol , Cytoplasmic vesicle , secretory vesicle , Secreted , Endoplasmic reticulum , Golgi apparatus

Background

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105175_39827_IFA_19011506_519.jpg

GTX105175 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1(GTX105175) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX105175_39827_IHC_19011506_873.jpg

GTX105175 IHC-P Image

AIMP1 antibody detects SCYE1 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
AIMP1 antibody (GTX105175) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105175_WB_19011506_725.jpg

GTX105175 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
12% SDS PAGE
GTX105175 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf