APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC , BCL-1 , HeLa , Jurkat
Predict Reactivity
Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human AIMP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10621475
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aminoacyl tRNA synthetase complex interacting multifunctional protein 1 , EMAP2 , EMAPII , HLD3 , SCYE1 , p43
Cellular Localization
Nucleus , Cytoplasm , cytosol , Cytoplasmic vesicle , secretory vesicle , Secreted , Endoplasmic reticulum , Golgi apparatus
Background
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115209 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX115209 diluted at 1:1000
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GTX115209 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
B: JurKat
10% SDS PAGE
GTX115209 diluted at 1:1000
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GTX115209 ICC/IF Image
AIMP1 antibody detects SCYE1 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SCYE1 protein stained by AIMP1 antibody (GTX115209) diluted at 1:500.
Blue: Hoechst 33343 staining.
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GTX115209 IHC-P Image
AIMP1 antibody detects AIMP1 protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
AIMP1 antibody (GTX115209) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115209 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using AIMP1(GTX115209) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115209 IHC-P Image
AIMP1 antibody detects AIMP1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
AIMP1 antibody (GTX115209) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for AIMP1 antibody (GTX115209). Be the first to share your publications with this product.
REVIEW
AIMP1 antibody
Cat. No. GTX115209
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Rating
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( Average 5 based on 1 users reviews)
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Application
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IHC (Whole mount)(IHC-Wm)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 20-Aug-2018
Application Tested :
IHC-Wm
Sample Species :
Dm
Sample :
Drosophila eye
Fixation :
4% PFA
Primary Antibody :
1:50, 4°C, 16Hr
