GeneTex
United States (US)

AIMP1 antibody

GTX115209_40401_IFA_18121410_110.jpg
GTX115209_40254_IHC_18121410_718.jpg
GTX115209_40254_WB_M_18121410_428.jpg
GTX115209_40254_IHC_M_18121410_458.jpg
GTX115209_WB_18121410_692.jpg
GTX115209_40254_20160125_IHC-P_18121410_469.jpg

Cat No. GTX115209

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Wm
Reactivity Human, Mouse, Drosophila
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HeLa , Jurkat , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AIMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aminoacyl Trna Synthetase Complex Interacting Multifunctional Protein 1,Emap2,Emapii,Hld3,Scye1,P43,Aimp1

Cellular Localization

Nucleus , Cytoplasm , cytosol , Cytoplasmic vesicle , secretory vesicle , Secreted , Endoplasmic reticulum , Golgi apparatus

Background

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115209_40401_IFA_18121410_110.jpg

GTX115209 ICC/IF Image

AIMP1 antibody detects SCYE1 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SCYE1 protein stained by AIMP1 antibody (GTX115209) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX115209_40254_IHC_18121410_718.jpg

GTX115209 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using AIMP1(GTX115209) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115209_40254_WB_M_18121410_428.jpg

GTX115209 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX115209 diluted at 1:1000

GTX115209_40254_IHC_M_18121410_458.jpg

GTX115209 IHC-P Image

AIMP1 antibody detects AIMP1 protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
AIMP1 antibody (GTX115209) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115209_WB_18121410_692.jpg

GTX115209 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: JurKat
10% SDS PAGE
GTX115209 diluted at 1:1000

GTX115209_40254_20160125_IHC-P_18121410_469.jpg

GTX115209 IHC-P Image

AIMP1 antibody detects AIMP1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
AIMP1 antibody (GTX115209) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

AIMP1 antibody Cat No. GTX115209

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Aug-2018
Score :
Application Tested : IHC-Wm
Sample Species : Dm
Sample : Drosophila eye
Fixation : 4% PFA
Primary Antibody : 1:50, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169