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AIRE (isoform 1) antibody, Internal

Anti-AIRE (isoform 1) antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89336
Anti-AIRE (isoform 1) antibody, Internal used in Western Blot (WB). GTX89336

Cat No. GTX89336

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Pig
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1μg/ml
IHC-P 2.5μg/ml

Note :

IHC-P
Human Thymus shows nuclear staining in select thymocytes. This product was confirmed in IHC on fetal Pig Thymus (PMID: 24770647).

Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89336-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_000374.1) only.

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autoimmune regulator , AIRE1 , APECED , APS1 , APSI , PGA1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012]

Database

DATA IMAGES

Anti-AIRE (isoform 1) antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89336

GTX89336 IHC-P Image

IHC-P analysis of human thymus using GTX89336 AIRE (isoform 1) antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-AIRE (isoform 1) antibody, Internal used in Western Blot (WB). GTX89336

GTX89336 WB Image

WB analysis of HEK293 cell lysates untransfected (Lane 3) and transfected with human AIRE (lane 4) using GTX89336 AIRE (isoform 1) antibody, Internal.
Dilution : 0.05μg/ml

REFERENCE

There are currently no references for AIRE (isoform 1) antibody, Internal (GTX89336). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AIRE (isoform 1) antibody, Internal (GTX89336). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 369