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AIRE (isoforms 1 + 2) antibody, Internal

Anti-AIRE (isoforms 1 + 2) antibody, Internal used in Western Blot (WB). GTX89216
Anti-AIRE (isoforms 1 + 2) antibody, Internal used in Western Blot (WB). GTX89216

Cat. No. GTX89216

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all AIRE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89216-PEP).

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KAKPPKKPESSAEQ, from the internal region of the protein sequence according to NP_000374.1; NP_000649.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autoimmune regulator , AIRE1 , APECED , APS1 , APSI , PGA1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012]

Database

DATA IMAGES

Anti-AIRE (isoforms 1 + 2) antibody, Internal used in Western Blot (WB). GTX89216

GTX89216 WB Image

WB analysis of human spleen lysate using GTX89216 AIRE (isoforms 1 + 2) antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-AIRE (isoforms 1 + 2) antibody, Internal used in Western Blot (WB). GTX89216

GTX89216 WB Image

WB analysis of HEK293 cell lysates untransfected (Lane 5 and 7) and transfected with human AIRE (lane 6) or mouse AIRE (lane 8) using GTX89216 AIRE (isoforms 1 + 2) antibody, Internal.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399