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AIRE (isoforms 1 and 2) antibody, C-term

Anti-AIRE (isoforms 1 and 2) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX13573
Anti-AIRE (isoforms 1 and 2) antibody, C-term used in Western Blot (WB). GTX13573

Cat No. GTX13573

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.5μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX13573-PEP).

Product Note

This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autoimmune regulator , AIRE1 , APECED , APS1 , APSI , PGA1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012]

Database

DATA IMAGES

Anti-AIRE (isoforms 1 and 2) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX13573

GTX13573 IHC-P Image

IHC-P analysis of human thymus using GTX13573 AIRE (isoforms 1 and 2) antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml

Anti-AIRE (isoforms 1 and 2) antibody, C-term used in Western Blot (WB). GTX13573

GTX13573 WB Image

WB analysis of human lymph node lysate using GTX13573 AIRE (isoforms 1 and 2) antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for AIRE (isoforms 1 and 2) antibody, C-term (GTX13573). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AIRE (isoforms 1 and 2) antibody, C-term (GTX13573). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339