GeneTex
United States (US)

AKAP 82 antibody

Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31595
Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31595
Anti-AKAP 82 antibody used in Western Blot (WB). GTX31595

Cat No. GTX31595

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

Human Testis Tissue Lysate(GTX15374) , Human Testis Tissue Slide(GTX21748)

Specificity/Sensitivity

AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

AKAP 82 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP 82.The immunogen is located within the first 50 amino acids of AKAP 82.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

A-Kinase Anchoring Protein 4,Akap 82,Akap-4,Akap82,Ct99,Fsc1,Hi,Prka4,Hakap82,P82,Akap4

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31595

GTX31595 IHC-P Image

IHC-P analysis of human testis tissue using GTX31595 AKAP 82 antibody.
Working concentration : 5 μg/ml

Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31595

GTX31595 IHC-P Image

IHC-P analysis of human testis tissue using GTX31595 AKAP 82 antibody.
Working concentration : 20 μg/ml

Anti-AKAP 82 antibody used in Western Blot (WB). GTX31595

GTX31595 WB Image

WB analysis of human testis tissue lysate using GTX31595 AKAP 82 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Package List Price ($)
$ 329