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AKAP 82 antibody

Anti-AKAP 82 antibody used in Western Blot (WB). GTX66294
Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX66294
Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX66294

Cat No. GTX66294

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

A-kinase anchoring protein 4 , AKAP 82 , AKAP-4 , AKAP82 , CT99 , FSC1 , HI , PRKA4 , hAKAP82 , p82

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AKAP 82 antibody used in Western Blot (WB). GTX66294

GTX66294 WB Image

WB analysis of various sample lysates using GTX66294 AKAP 82 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX66294

GTX66294 IHC-P Image

IHC-P analysis of rat testis tissue using GTX66294 AKAP 82 antibody.
Blue : DAPI
Dilution : 1:100

Anti-AKAP 82 antibody used in IHC (Paraffin sections) (IHC-P). GTX66294

GTX66294 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX66294 AKAP 82 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf