*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A549 , HeLa , HepG2
Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS (pH7), 1% BSA, 20% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human AKAP1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
A-kinase anchoring protein 1 , AKAP , AKAP121 , AKAP149 , AKAP84 , D-AKAP1 , PPP1R43 , PRKA1 , SAKAP84 , TDRD17
Mitochondrion outer membrane
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq]
GTX115067 WB Image
Wild-type (WT) and AKAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKAP1 antibody [N1N2], N-term (GTX115067) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX115067 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using AKAP1(GTX115067) antibody at 1:200 dilution.
GTX115067 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKAP1(GTX115067) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX115067 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX115067 diluted at 1:10000