GeneTex

  • Country / Location Selection

United States (US)

AKAP1 antibody [N1N2], N-term

KO/KD
Anti-AKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115067
Anti-AKAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115067
Anti-AKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115067
Anti-AKAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115067

Cat. No. GTX115067

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AKAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HeLa , 293T

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human AKAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724107

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

A-kinase anchoring protein 1 , AKAP , AKAP121 , AKAP149 , AKAP84 , D-AKAP1 , PPP1R43 , PRKA1 , SAKAP84 , TDRD17

Cellular Localization

Mitochondrion outer membrane

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115067

GTX115067 WB Image

KO/KD

Wild-type (WT) and AKAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKAP1 antibody [N1N2], N-term (GTX115067) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115067

GTX115067 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKAP1(GTX115067) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115067

GTX115067 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX115067 diluted at 1:10000
Anti-AKAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115067

GTX115067 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using AKAP1(GTX115067) antibody at 1:200 dilution.

REFERENCE

There are currently no references for AKAP1 antibody [N1N2], N-term (GTX115067). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for AKAP1 antibody [N1N2], N-term (GTX115067). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169