GeneTex
United States (US)

AKAP10 antibody [C3], C-term

GTX106430_IHC1_19011506_375.jpg
GTX106430_39890_IFA_19011506_155.jpg
GTX106430_39890_20160720_IHC-P_R_19011506_298.jpg
GTX106430_39890_20160720_IHC-P_M_19011506_368.jpg
GTX106430_IHC2_19011506_211.jpg

Cat No. GTX106430

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

Synthetic peptide: 1:8,000

Predict Reactivity

(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

D-AKAP2 antibody, MGC9414 antibody, PRKA10 antibody, AKAP10 antibody, dual specificity A kinase-anchoring protein 2 antibody, D-AKAP-2 antibody, a kinase anchor protein 10, mitochondrial antibody, A-kinase anchor protein 10, mitochondrial antibody, dual-specificity A-kinase anchoring protein 2 antibody, protein kinase A anchoring protein 10 antibody, protein kinase A-anchoring protein 10 antibody, AKAP-10 antibody, mitochondrial A kinase PPKA anchor protein 10 antibody, A kinase (PRKA) anchor protein 10 antibody

Cellular Localization

Mitochondrion , Membrane , Cytoplasm

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq]
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Database

DATA IMAGES
GTX106430_IHC1_19011506_375.jpg

GTX106430 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKAP10(GTX106430) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106430_39890_IFA_19011506_155.jpg

GTX106430 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using AKAP10(GTX106430) antibody at 1:500 dilution.

GTX106430_39890_20160720_IHC-P_R_19011506_298.jpg

GTX106430 IHC-P Image

AKAP10 antibody [C3], C-term detects AKAP10 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
AKAP10 antibody [C3], C-term (GTX106430) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106430_39890_20160720_IHC-P_M_19011506_368.jpg

GTX106430 IHC-P Image

AKAP10 antibody [C3], C-term detects AKAP10 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AKAP10 antibody [C3], C-term (GTX106430) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106430_IHC2_19011506_211.jpg

GTX106430 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human OVCAR3, using AKAP10(GTX106430) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169