GeneTex
United States (US)

AKAP10 antibody, Internal

GTX88680_2175_WB_18122102_509.jpg

Cat No. GTX88680

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88680-PEP).

Predict Reactivity

Human, Mouse, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RKVKGKEQEKTSD, from the internal region of the protein sequence according to NP_009133.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

A-Kinase Anchoring Protein 10,Akap-10,D-Akap-2,D-Akap2,Prka10,Akap10

Cellular Localization

Predominantly mitochondrial but also membrane associated and cytoplasmic.

Background

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins bind to the regulatory subunits of protein kinase A (PKA) and confine the holoenzyme to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. Polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. [provided by RefSeq, May 2012]

Database

DATA IMAGES
GTX88680_2175_WB_18122102_509.jpg

GTX88680 WB Image

WB analysis of rat brain lysate using GTX88680 AKAP10 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329