GeneTex
United States (US)

AKAP12 antibody [C3], C-term

GTX106373_WB_19011506_365.jpg
GTX106373_IHC_19011506_712.jpg
GTX106373_IFA_19011506_385.jpg

Cat No. GTX106373

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

191 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKAP250 antibody, DKFZp686M0430 antibody, DKFZp686O0331 antibody, FLJ20945 antibody, FLJ97621 antibody, AKAP12 antibody, Src-Suppressed C Kinase Substrate antibody, "A-kinase anchor protein, 250kDa antibody", myasthenia gravis autoantigen gravin antibody, A-kinase anchor protein 12 antibody, kinase scaffold protein gravin antibody, AKAP 250 antibody, A kinase (PRKA) anchor protein 12 antibody

Cellular Localization

Cytoplasm , cell cortex , cytoskeleton

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

DATA IMAGES
GTX106373_WB_19011506_365.jpg

GTX106373 WB Image

Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
5% SDS PAGE
GTX106373 diluted at 1:1000

GTX106373_IHC_19011506_712.jpg

GTX106373 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using AKAP12(GTX106373) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106373_IFA_19011506_385.jpg

GTX106373 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12(GTX106373) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169