AKAP12 antibody [C3], C-term

Name: AKAP12 antibody [C3], C-term
Brand: GeneTex
SKU: GTX106373
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-AKAP12 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106373

Anti-AKAP12 antibody [C3], C-term used in Western Blot (WB). GTX106373

Anti-AKAP12 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106373

Cat. No. GTX106373

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all AKAP12 products

View product citations for antibody GTX106373 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

191 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949619

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

A-kinase anchoring protein 12 , AKAP250 , SSeCKS

Cellular Localization

Cytoplasm , cell cortex , cytoskeleton

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Gene ID: 9590 AKAP12
UniProt: Q02952 AKAP12

Research Area

DATA IMAGES

GTX106373 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12(GTX106373) antibody at 1:200 dilution.

GTX106373 WB Image

Boiled and unboiled whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AKAP12 antibody [C3], C-term (GTX106373) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

GTX106373 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using AKAP12(GTX106373) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106373 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AKAP12 antibody [C3] (GTX106373) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Peng Zhao et al. Chemico-Biological Interactions 2016; 245 : 39-49 Label-free quantitative proteomic analysis of benzo(a)pyrene-transformed 16HBE cells serum-free culture supernatant and xenografted nude mice sera.

Application : WB

Reactivity : Human

Hammond DE et al. J Proteome Res 2015; 14 (3) : 1535-46 Differential Reprogramming of Isogenic Colorectal Cancer Cells by Distinct Activating KRAS Mutations.

Application : WB

Reactivity : Human

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