GeneTex
  • Country / Location Selection

United States (US)

AKAP12 antibody [C3], C-term

Anti-AKAP12 antibody [C3], C-term used in Western Blot (WB). GTX106373
Anti-AKAP12 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106373
Anti-AKAP12 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106373

Cat No. GTX106373

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

191 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949619

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

A-kinase anchoring protein 12 , AKAP250 , SSeCKS

Cellular Localization

Cytoplasm , cell cortex , cytoskeleton

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKAP12 antibody [C3], C-term used in Western Blot (WB). GTX106373

GTX106373 WB Image

Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
5% SDS PAGE
GTX106373 diluted at 1:1000

Anti-AKAP12 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106373

GTX106373 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using AKAP12(GTX106373) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AKAP12 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106373

GTX106373 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12(GTX106373) antibody at 1:200 dilution.

REFERENCE

REVIEW

AKAP12 antibody [C3], C-term Cat No. GTX106373

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 12-Jun-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVISE cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Non-specific siRNA transfected. Lane 2: AKAP12-specific siRNA transfected.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169