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AKAP12 antibody

Anti-AKAP12 antibody used in Western Blot (WB). GTX80649

Cat. No. GTX80649

Host

 Sheep

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, IHC

Reactivity

 Rat
Package
125 μg ($399)
See all AKAP12 products

APPLICATION

Application Note

Recommended Starting Dilutions:
IP: Use at a concentration of 2-5 μg/mL
WB: Use at a concentration of 2-5 μg/mL
IHC: Use at an assay-dependent dilution
Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

181 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.08% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat AKAP12 fragment corresponding to amino acid 620-1596.

Purification

Ammonium sulfate purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

A-kinase anchoring protein 12 , AKAP12A , AKAP12B , AKAP12G

Cellular Localization

Cytoplasm, cytoskeleton , Membrane

Database

Research Area

DATA IMAGES

Anti-AKAP12 antibody used in Western Blot (WB). GTX80649

GTX80649 WB Image

Total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyzed. The membrane was probed with 5 μg/ml of pre-immune serum or 5 μg of SSecks antibody (3 second exposure shown). The blot was then washed and probed with monoclonal anti-sheep IgG antibody coupled to horseradish peroxidase 1:10,000, and detected with luminol.

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399