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AKAP5 antibody

Anti-AKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87532
Anti-AKAP5 antibody used in Western Blot (WB). GTX87532

Cat. No. GTX87532

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all AKAP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human AKAP5 (1-50).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AKAP5 , AKAP75 , AKAP79 , Akinase anchoring protein 5 , H21 , A-kinase anchoring protein 5

Cellular Localization

Membrane

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87532

GTX87532 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX87532 AKAP5 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP5 antibody used in Western Blot (WB). GTX87532

GTX87532 WB Image

WB analysis of HepG2, HeLa, and COLO205 cell lysates using GTX87532 AKAP5 antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399