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AKAP8 antibody

Anti-AKAP8 antibody used in Western Blot (WB). GTX32429
Anti-AKAP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32429

Cat. No. GTX32429

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all AKAP8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human AKAP8

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

A-kinase anchoring protein 8 , AKAP 95 , AKAP-8 , AKAP-95 , AKAP95

Cellular Localization

Nucleus,Cytoplasm,Nucleolus

Background

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-AKAP8 antibody used in Western Blot (WB). GTX32429

GTX32429 WB Image

WB analysis of various sample lysates using GTX32429 AKAP8 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-AKAP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32429

GTX32429 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32429 AKAP8 antibody.
Blue : DAPI
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399