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AKAP8L antibody [N1N3]

KO/KD
Anti-AKAP8L antibody [N1N3] used in Western Blot (WB). GTX115831

Cat. No. GTX115831

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AKAP8L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKAP8L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622099

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

A-kinase anchoring protein 8 like , HA95 , HAP95 , NAKAP , NAKAP95

Background

Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta.

Database

Research Area

DATA IMAGES

Anti-AKAP8L antibody [N1N3] used in Western Blot (WB). GTX115831

GTX115831 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX115831 diluted at 1:5000

REFERENCE

Application Reference

Zhang S et al. PLoS Genet 2021; 17 (10) : e1009832 AKAP13 couples GPCR signaling to mTORC1 inhibition.
Application : WB
Reactivity : Human
Melick CH et al. J Biol Chem 2020; 295 (23) : 8096-8105 A-kinase anchoring protein 8L interacts with mTORC1 and promotes cell growth.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, IP
Reactivity : Human
Chen JY et al. J Nutr Biochem 2013; 24 (11) : 1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : ELISA
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169