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AKR1A1 antibody [6E3]

Anti-AKR1A1 antibody [6E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84913
Anti-AKR1A1 antibody [6E3] used in Flow cytometry (FACS). GTX84913
Anti-AKR1A1 antibody [6E3] used in Flow cytometry (FACS). GTX84913
Anti-AKR1A1 antibody [6E3] used in Western Blot (WB). GTX84913
Anti-AKR1A1 antibody [6E3] used in Flow cytometry (FACS). GTX84913
Anti-AKR1A1 antibody [6E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84913
Anti-AKR1A1 antibody [6E3] used in IHC (Paraffin sections) (IHC-P). GTX84913

Cat. No. GTX84913

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6E3

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all AKR1A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AKR1A1 (NP_006057) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member A1 , ALDR1 , ALR , ARM , DD3 , HEL-S-6

Cellular Localization

Cytoplasm, cytosol,Apical cell membrane

Background

Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).

Database

Research Area

DATA IMAGES

Anti-AKR1A1 antibody [6E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84913

GTX84913 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84913 AKR1A1 antibody [6E3].

Anti-AKR1A1 antibody [6E3] used in Flow cytometry (FACS). GTX84913

GTX84913 FACS Image

FACS analysis of HeLa cells using GTX84913 AKR1A1 antibody [6E3].
Red : Primary antibody
Blue : Negative control antibody

Anti-AKR1A1 antibody [6E3] used in Flow cytometry (FACS). GTX84913

GTX84913 FACS Image

FACS analysis of HEK293T cells transfected with either AKR1A1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84913 AKR1A1 antibody [6E3].

Anti-AKR1A1 antibody [6E3] used in Western Blot (WB). GTX84913

GTX84913 WB Image

WB analysis of HEK293T cells transfected with AKR1A1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84913 AKR1A1 antibody [6E3].
Loading : 5 ug per lane

Anti-AKR1A1 antibody [6E3] used in Flow cytometry (FACS). GTX84913

GTX84913 FACS Image

FACS analysis of Jurkat cells using GTX84913 AKR1A1 antibody [6E3].
Red : Primary antibody
Blue : Negative control antibody

Anti-AKR1A1 antibody [6E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84913

GTX84913 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with AKR1A1 plasmid using GTX84913 AKR1A1 antibody [6E3].

Anti-AKR1A1 antibody [6E3] used in IHC (Paraffin sections) (IHC-P). GTX84913

GTX84913 IHC-P Image

IHC-P analysis of liver tissue using GTX84913 AKR1A1 antibody [6E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

Application Reference

Alfonso Sequeda-JuÁre et al. Anticancer Res. 2020; 40 (11) : 6273-6284 Use of AKR1C1 and TKTL1 in the Diagnosis of Low-grade Squamous Intraepithelial Lesions from Mexican Women.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519