AKR1B1 antibody [N1C3]

Cat. No. GTX113381

Reference 9

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Rat, Pig
Package	100 μl ($399), 25 μl ($169)

See all AKR1B1 products

View product citations for antibody GTX113381 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rabbit, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKR1B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036204

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member B , ADR , ALDR1 , ALR2 , AR

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq]

Database

Gene ID: 231 AKR1B1
UniProt: P15121 AKR1B1

Research Area

DATA IMAGES

GTX113381 WB Image

Wild-type (WT) and AKR1B1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1B1 antibody [N1C3] (GTX113381) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113381 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1B1 antibody [N1C3] (GTX113381) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX113381 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKR1B1(GTX113381) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min