APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse colon , rat colon
Product Note
Based on sequence homology, this antibody may cross react with AKR1B15.
Predict Reactivity
Rabbit(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human AKR1B10 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949620
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aldo-keto reductase family 1 member B10 , AKR1B11 , AKR1B12 , ALDRLn , ARL-1 , ARL1 , HIS , HSI
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109571 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with AKR1B10 antibody (GTX109571) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109571 WB Image
AKR1B10 antibody detects AKR1B10 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
10% SDS-PAGE
AKR1B10 antibody (GTX109571) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109571 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10(GTX109571) antibody at 1:200 dilution.
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GTX109571 IHC-P Image
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10(GTX109571) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109571 WB Image
AKR1B10 antibody detects AKR1B10 protein by western blot analysis.
A. 50 μg mouse colon lysate/extract
10% SDS-PAGE
AKR1B10 antibody (GTX109571) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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