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AKR1C1 antibody [AT6D10]

Anti-AKR1C1 antibody [AT6D10] used in Western Blot (WB). GTX53684

Cat No. GTX53684

Host

Mouse

Clonality

Monoclonal

Clone Name

AT6D10

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human AKR1C1 (1-323aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Aldo-Keto Reductase Family 1 Member C1 , 2-Alpha-Hsd , 20-Alpha-Hsd , C9 , Dd1 , Dd1/Dd2 , Ddh , Ddh1 , H-37 , Hakrc , Hbab , Mbab , Akr1C1

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AKR1C1 antibody [AT6D10] used in Western Blot (WB). GTX53684

GTX53684 WB Image

WB analysis of HepG2 lysate (33ug per lane) using AKR1C1 antibody at the indicated dilutions.

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf