GeneTex
United States (US)

AKR1C1 antibody

Overexpression
GTX105620_40555_WB_19011506_578.jpg
GTX105620_40002_WB_M_liver_19011506_614.jpg
GTX105620_42326_20151203_WB_19011506_597.jpg
GTX105620_40002_IFA_19011506_516.jpg
GTX105620_IHC_19011506_573.jpg
GTX105620_40555_20180831_WB_B_19011506_197.jpg

Cat No. GTX105620

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , A549 , mouse liver

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

2-ALPHA-HSD antibody, 20-ALPHA-HSD antibody, C9 antibody, DD1 antibody, DDH antibody, DDH1 antibody, H-37 antibody, HAKRC antibody, MBAB antibody, MGC8954 antibody, AKR1C1 antibody, 20-alpha-hydroxysteroid dehydrogenase antibody, dihydrodiol dehydrogenase 1/2 antibody, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody, high-affinity hepatic bile acid-binding protein antibody, dihydrodiol dehydrogenase isoform DD1 antibody, indanol dehydrogenase antibody, aldo-keto reductase family 1 member C1 antibody, aldo-keto reductase C antibody, type II 3-alpha-hydroxysteroid dehydrogenase antibody, chlordecone reductase homolog HAKRC antibody, hepatic dihydrodiol dehydrogenase antibody, 20 alpha-hydroxysteroid dehydrogenase antibody, dihydrodiol dehydrogenase 1 antibody, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX105620_40555_WB_19011506_578.jpg

GTX105620 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX105620 diluted at 1:1000

GTX105620_40002_WB_M_liver_19011506_614.jpg

GTX105620 WB Image

AKR1C1 antibody detects AKR1C1 protein by western blot analysis.
A. 50 µg mouse liver lysate/extract
10% SDS-PAGE
AKR1C1 antibody (GTX105620) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105620_42326_20151203_WB_19011506_597.jpg

GTX105620 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000.

GTX105620_40002_IFA_19011506_516.jpg

GTX105620 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(GTX105620) antibody at 1:200 dilution.

GTX105620_IHC_19011506_573.jpg

GTX105620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(GTX105620) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105620_40555_20180831_WB_B_19011506_197.jpg

GTX105620 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf