GeneTex
United States (US)

AKR1C3 antibody [C2C3], C-term

GTX104627_39624_20181221_WB_shRNA_watermark_19011519_133.jpg
GTX104627_39624_WB_R_liver_19011506_647.jpg
GTX104627_WB_19011506_457.jpg
GTX104627_39624_20150529_IP_19011506_156.jpg
GTX104627_IFA_19011506_865.jpg
GTX104627_39624_IHC_19011506_144.jpg

Cat No. GTX104627

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HepG2 , HeLa , AKR1C3shRNA-transfected HeLa , rat liver
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKR1C3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldo-Keto Reductase Family 1 Member C3,Dd3,Ddx,Ha1753,Hakrb,Hakre,Hsd17B5,Pgfs,Hlupgfs,Akr1C3

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104627_39624_20181221_WB_shRNA_watermark_19011519_133.jpg

GTX104627 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104627_39624_WB_R_liver_19011506_647.jpg

GTX104627 WB Image

AKR1C3 antibody [C2C3], C-term detects AKR1C3 protein by western blot analysis.
A. 50 µg rat liver lysate/extract
10% SDS-PAGE
AKR1C3 antibody [C2C3], C-term (GTX104627) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104627_WB_19011506_457.jpg

GTX104627 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX104627 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104627_39624_20150529_IP_19011506_156.jpg

GTX104627 IP Image

Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 μg of AKR1C3 antibody [C2C3], C-term (GTX104627).
Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term (GTX104627).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104627_IFA_19011506_865.jpg

GTX104627 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1C3(GTX104627) antibody at 1:200 dilution.

GTX104627_39624_IHC_19011506_144.jpg

GTX104627 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3(GTX104627) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169