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AKR1C3 antibody [C2C3], C-term

KO/KD
Anti-AKR1C3 antibody [C2C3], C-term used in Western Blot (WB). GTX104627
Anti-AKR1C3 antibody [C2C3], C-term used in Western Blot (WB). GTX104627
Anti-AKR1C3 antibody [C2C3], C-term used in Western Blot (WB). GTX104627
Anti-AKR1C3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104627
Anti-AKR1C3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104627
Anti-AKR1C3 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104627

Cat. No. GTX104627

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all AKR1C3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

rat liver , HepG2 , HeLa

Product Note

Based on sequence homology, this antibody may cross react with other family members.

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKR1C3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240438

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member C3 , DD3 , DDX , HA1753 , HAKRB , HAKRe , HSD17B5 , PGFS , hluPGFS

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKR1C3 antibody [C2C3], C-term used in Western Blot (WB). GTX104627

GTX104627 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKR1C3 antibody [C2C3], C-term used in Western Blot (WB). GTX104627

GTX104627 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKR1C3 antibody [C2C3], C-term used in Western Blot (WB). GTX104627

GTX104627 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX104627 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKR1C3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104627

GTX104627 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1C3(GTX104627) antibody at 1:200 dilution.

Anti-AKR1C3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104627

GTX104627 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3(GTX104627) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AKR1C3 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104627

GTX104627 IP Image

Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 μg of AKR1C3 antibody [C2C3], C-term (GTX104627).
Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term (GTX104627).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169