GeneTex
United States (US)

AKR7A2 antibody

GTX104299_IFA_19011506_543.jpg
GTX104299_39785_IHC_19011506_261.jpg
GTX104299_WB_19011506_342.jpg
GTX104299_39785_IHC_M_19011506_227.jpg

Cat No. GTX104299

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKR7A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldo-Keto Reductase Family 7 Member A2,Afar,Afar1,Afb1-Ar1,Akr7,Akr7A2

Cellular Localization

Golgi apparatus

Background

Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX104299_IFA_19011506_543.jpg

GTX104299 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR7 (GTX104299) antibody at 1:200 dilution.

GTX104299_39785_IHC_19011506_261.jpg

GTX104299 IHC-P Image

AKR7A2 antibody detects AKR7A2 protein at cytosol on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep2 xenograft.
AKR7A2 antibody (GTX104299) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104299_WB_19011506_342.jpg

GTX104299 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX104299 diluted at 1:1000

GTX104299_39785_IHC_M_19011506_227.jpg

GTX104299 IHC-P Image

AKR7A2 antibody detects AKR7A2 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
AKR7A2 antibody (GTX104299) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169