GeneTex

  • Country / Location Selection

United States (US)

AKT antibody [HL2915]

Recombinant antibody Overexpression
Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258
Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258
Anti-AKT antibody [HL2915] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640258
Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258
Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258
Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258
Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258
Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258

Cat. No. GTX640258

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2915

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all AKT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Observed MW

60 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human AKT.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Early endosome,Membrane

Database

Research Area

DATA IMAGES

Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258

GTX640258 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258

GTX640258 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT antibody [HL2915] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640258

GTX640258 ICC/IF Image

AKT antibody [HL2915] detects AKT protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258

GTX640258 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258

GTX640258 IHC-P Image

AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat esophagus.
AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258

GTX640258 IHC-P Image

AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258

GTX640258 IHC-P Image

AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258

GTX640258 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for AKT antibody [HL2915] (GTX640258). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for AKT antibody [HL2915] (GTX640258). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 419
$ 169
Custom Conjugations