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AKT antibody [N3C2], Internal

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Immunohistochemistry (IHC). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

Cat No. GTX121937

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , NIH-3T3 , Raw264.7 , JC , BCL-1, PC-12 , C2C12 , HA-human Akt -transfected HeLa , *mouse melanoma

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377414)

Predict Reactivity

Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, AKT1 antibody, protein kinase B alpha antibody, rac protein kinase alpha antibody, RAC-alpha serine/threonine-protein kinase antibody, proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, PKB alpha antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, AKT2 antibody, protein kinase B beta antibody, murine thymoma viral (v-akt) homolog-2 antibody, protein kinase Akt-2 antibody, RAC-PK-beta antibody, PKB beta antibody, rac protein kinase beta antibody, RAC-beta serine/threonine-protein kinase antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, DKFZp434N0250 antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AKT3 antibody, PKB gamma antibody, RAC-gamma serine/threonine-protein kinase antibody, RAC-gamma serine/threonine protein kinase antibody, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Research Area

DATA IMAGES
Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Sample (30 µg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX121937 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX121937

GTX121937 IP Image

Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal (GTX121937).
Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX121937 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT antibody [N3C2], Internal used in Immunohistochemistry (IHC). GTX121937

GTX121937 IHC Image

Staining of Human normal esophagus tissue sections using anti-Akt1/2/3 antibody (GTX121937).

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AKT antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121937

GTX121937 ICC/IF Image

Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Akt1 protein stained by Akt1/2/3 antibody (GTX121937) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AKT antibody [N3C2], Internal used in Western Blot (WB). GTX121937

GTX121937 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
REVIEW

AKT antibody [N3C2], Internal Cat No. GTX121937

Rating ( Average 4 based on 4 users reviews)
Application
Western Blot(WB) ( Average 4 based on 4 users reviews)
Date : Anonymous submitted on 10-Apr-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 1.66 ug
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Raw264.7 transfected pcDNA3.1(+) whole cell lysate, Lane 2: Raw264.7 transfected pCA-AKT whole cell lysate
Date : Anonymous submitted on 13-Dec-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 20 μg
Blocking : 1% BSA in TBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 10°C, 12Hr
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 26-Mar-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : melanoma cells
Amount used : 40μg
Blocking : 5 % MTBST ( 2.5 g non-fat milk + 50 mL 1x TBST), 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169