GeneTex
United States (US)

AKT antibody

GTX28805_20160330_WB_1_18121410_743.jpg
GTX28805_20160330_IHC-P_18121410_873.jpg
GTX28805_20160330_ICCIF_18121410_569.jpg
GTX28805_20160330_WB_18121410_417.jpg
AKT-antibody__GTX28805-1_18121409_116.jpg

Cat No. GTX28805

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human, Mouse, Rat, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:100 - 1:1000
IHC-P 1:500 - 1:2000
FACS Assay dependent
ELISA 1:2000 - 1:10000
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH usin

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways (reviewed in ref 1). Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation (reviewed in 2). Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Research Area

DATA IMAGES
GTX28805_20160330_WB_1_18121410_743.jpg

GTX28805 WB Image

Western Blot of simultaneous detection of unphosphorylated and phosphorylated Rabbit Anti-AKT antibody (GTX28805). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Load: 35 µg per lane. Primary antibody: rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:1000 for overnight at 4°C. Secondary antibody: Flourescent 549 conjugated anti-rabbit IgG (green) and flourescent 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.

GTX28805_20160330_IHC-P_18121410_873.jpg

GTX28805 IHC-P Image

Immunohistochemical analysis of formalin-fixed paraffin-embedded tissue, A: Normal colon tissue, B: Colon tumor tissue, using AKT(GTX28805) antibody at 1:1,000 dilution for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT is nuclear. Staining: AKT as precipitated red signal with hematoxylin purple nuclear counterstain.

GTX28805_20160330_ICCIF_18121410_569.jpg

GTX28805 ICC/IF Image

ICC/IF of the Akt antibody is used at 1: 80 for 1hr at RT on cultured neonatal rat cardiomyocytes (Green). The red is Texas-red phalloidin that labels actin filaments.

GTX28805_20160330_WB_18121410_417.jpg

GTX28805 WB Image

Western blot using Akt antibody at 1:500 for overnight at 4°C on 20 ug NIH/3T3 whole cell lysate.

AKT-antibody__GTX28805-1_18121409_116.jpg

GTX28805 Image


REFERENCE
Package List Price ($)
$ 289