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AKT antibody

Anti-AKT antibody used in Western Blot (WB). GTX48651

Cat No. GTX48651

Host

Sheep

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, ELISA, IHC

Reactivity

Human
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
FACS Assay dependent
ELISA 1:5000 - 1:20000
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein.  A residue of cysteine was added to facilitate coupling.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability.Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Research Area

DATA IMAGES

Anti-AKT antibody used in Western Blot (WB). GTX48651

GTX48651 WB Image

Anti-AKT Antibody in western blot showing detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. AKT antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4º C. IRDYE800 conjugated Dnky-anti-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf