WB analysis of various samples using GTX48651 AKT antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : H2O2-treated MCF-7 whole cell lysate
Lane 3 : Recombinant AKT protein
Lane 4 : A431 whole cell lysate
Loading : 35 μg
Dilution : 1:300

WB analysis of various samples using GTX48651 AKT antibody.
Lane 1 : Protein ladder
Lane 2 : Recombinant AKT1 protein
Lane 3 : Recombinant AKT2 protein
Lane 3 : Recombinant AKT3 protein
Loading : 50 ng
Dilution : 1:1000

Anti-AKT Antibody in western blot showing detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. AKT antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4º C. IRDYE800 conjugated Dnky-anti-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature.