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AKT (phospho Ser473) antibody

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX28932
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932
Anti-AKT (phospho Ser473) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28932
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX28932

Cat No. GTX28932

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

For IHC-P: Use at a dilution of 1:100 - 1:500. No pre-treatment is required. For WB: Use at a dilution of 1:200 - 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

53 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.3, 50% Glycerol and 150mM NaCl containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: CHFPQFSpYSAS conjugated to KLH, corresponding to amino acids 460-480 of Human AKT.

Purification

Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactiv

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Akt Serine/Threonine Kinase 1,Akt,Cws6,Pkb,Pkb-Alpha,Prkba,Rac,Rac-Alpha,Akt1

Background

Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways (reviewed in ref 1). Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation (reviewed in 2). Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Database

Research Area

DATA IMAGES
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX28932

GTX28932 WB Image

Rabbit anti-AKT pS473 was used at a 1:200 dilution to detect phosphorylated AKT by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase was used.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932

GTX28932 IHC-P Image

Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT by immunohistochemistry in human breast tumor tissue.
The staining is much stronger than the weak basal level of phosphorylation in normal breast.Rabbit anti-AKT pS473 antibody was used with no pretreatment of tissue.Tissue was formalin-fixed and paraffin embedded.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932

GTX28932 IHC-P Image

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Akt (phospho-Ser473) antibody (GTX28932).

Anti-AKT (phospho Ser473) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28932

GTX28932 ICC/IF Image

Immunofluorescence Confocal Microscopy of Rabbit anti-AKT pS473 antibody (GTX28932). Tissue: cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:40 for 1 h at RT. Secondary antibody: texas-red conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear. Staining: AKT pS473 as green fluorescent signal with texas-red conjugated phalloidin (red) to label filamentous actin.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932

GTX28932 IHC-P Image

Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT by immunohistochemistry in normal human breast tissue. AKT is weakly phosphorylated in normal tissue in the breast. The phosphorylated AKT is clearly localized in the cytoplasm. Rabbit anti-AKT pS473 antibody was used with no pretreatment of tissue. Tissue was formalin-fixed and paraffin embedded.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX28932

GTX28932 IHC-P Image

This image is a magnification of the accompanying breast tumor image.

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX28932

GTX28932 WB Image

Western blot analysis of extract from HeLa cells untreated or treated with heat shock using Akt (phospho-Ser473) antibody (GTX28932, Lane 1 and 2).

REFERENCE
Application Reference
Package List Price ($)
$ 319