GeneTex
  • Country / Location Selection

United States (US)

AKT (phospho Ser473) antibody

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50186
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186

Cat No. GTX50186

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Specificity/Sensitivity

This antibody is raised against human AKT2 phosphorylated at Ser474. Based on sequence homology, it is predicted to react with AKT1 and AKT3 when phosphorylated at the corresponding residues - AKT1 Ser473 and AKT3 Ser472.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from human AKT.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Membrane

Database

Research Area

DATA IMAGES

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186

GTX50186 WB Image

WB analysis of extracts from 293 cells treated with serum or calf intestinal phosphatase (CIP) using GTX50186 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50186

GTX50186 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50186 AKT (phospho Ser473) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186

GTX50186 WB Image

WB analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GTX50186 AKT (phospho Ser473) antibody.

REFERENCE

There are currently no references for AKT (phospho Ser473) antibody (GTX50186). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AKT (phospho Ser473) antibody (GTX50186). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf