*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody is raised against human AKT2 phosphorylated at Ser474. Based on sequence homology, it is predicted to react with AKT1 and AKT3 when phosphorylated at the corresponding residues - AKT1 Ser473 and AKT3 Ser472.
Mouse, Rat(>80% identity)
PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from human AKT.
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2
GTX50186 WB Image
WB analysis of extracts from 293 cells treated with serum or calf intestinal phosphatase (CIP) using GTX50186 AKT (phospho Ser473) antibody.
GTX50186 IHC-P Image
IHC-P analysis of human lung carcinoma tissue using GTX50186 AKT (phospho Ser473) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
GTX50186 WB Image
WB analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GTX50186 AKT (phospho Ser473) antibody.