Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5-4 μg/ml |
10-20 μg/ml |
Not tested in other applications.
Product Note
The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2
and Akt3 when phosphorylated at equivalent sites.
Form
Liquid
Buffer
PBS, 1% BSA, 50% Glycerol
Preservative
0.02% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_10720029
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
thymoma viral proto-oncogene 1 , thymoma viral proto-oncogene 2 , thymoma viral proto-oncogene 3 , Akt , LTR-akt , PKB , PKB/Akt , PKBalpha , Rac , 2410016A19Rik , AW554154 , PKB , PKBbeta , AI851531 , D930002M15Rik , Nmf350
Cellular Localization
Cytoplasm,Nucleus,Cell membrane
Database
Research Area