AKT (phospho Thr308) antibody

Dot blot analysis of various BSA-conjugated peptides using GTX28933 AKT (phospho Thr308) antibody.
Row A: AKT
Row B : AKT (phospho Thr308)
Row C and D : AKT (phospho Ser473)
Row E and F : CDC27 (phospho Thr244)
Row G : BSA
Lane 1 : 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

WB analysis of GST-tagged AKT1 recombinant protein using GTX28933 AKT (phospho Thr308) antibody.
Lane 1 : In-active GST-tagged AKT1 recombinant protein
Lane 2 : Active GST-tagged AKT1 recombinant protein
Loading : 50 ng
Dilution : 1:1000

IHC-P analysis of human lung tissue using GTX28933 AKT (phospho Thr308) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

WB analysis of various samples using GTX28933 AKT (phospho Thr308) antibody.
Lane 1 : Protein ladder
Lane 2 : Human spleen tissue lysate
Lane 3 : Human small intestine tissue lysate
Lane 4 : Human placenta tissue lysate
Lane 5 : Human skeletal muscle tissue lysate
Lane 6 : Human cerebellum tissue lysate
Lane 7 : Human lung tissue lysate
Lane 8 : Human tonsil tissue lysate
Lane 9 : Human thymus tissue lysate
Lane 10 : Protein ladder
Dilution : 1:1000

IHC-P analysis of human testis tissue using GTX28933 AKT (phospho Thr308) antibody.
Antigen retrieval : Heat mediated Leica Bond Epitope Retrieval Buffer 1 (Citrate solution, pH 6)

IHC-P analysis of human breast tissue using GTX28933 AKT (phospho Thr308) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

ELISA analysis of Non-phospho and phospho peptide using serially diluted GTX28933 AKT (phospho Thr308) antibody.
Blue : Phospho
Purple : Non-phospho
Coating : 0.1 μg

Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4ºC. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.