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AKT (phospho Thr308) antibody

Orthogonal
Anti-AKT (phospho Thr308) antibody used in IHC (Paraffin sections) (IHC-P). GTX79150
Anti-AKT (phospho Thr308) antibody used in Western Blot (WB). GTX79150

Cat. No. GTX79150

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11163439

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT,AKT serine/threonine kinase 1,AKT1,CWS6,PKB,PKBALPHA,PRKBA,RAC,RACALPHA,AKT phospho

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-AKT (phospho Thr308) antibody used in IHC (Paraffin sections) (IHC-P). GTX79150

GTX79150 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX79150 AKT (phospho Thr308) antibody.

Anti-AKT (phospho Thr308) antibody used in Western Blot (WB). GTX79150

GTX79150 WB Image

Orthogonal

WB analysis of 293 cells treated with insulin lysate using GTX79150 AKT (phospho Thr308) antibody.

REFERENCE

Application Reference

Reilly L et al. iScience 2023; 26 (5) : 106623 Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis.
Wu YH et al. Front Pharmacol 2020; 11 : 206 Comparing PI3K/Akt Inhibitors Used in Ovarian Cancer Treatment.
Application : IHC-P
Reactivity : Human
Zemanovic S et al. Cell Rep 2018; 25 (9) : 2605-2616.e7 Dynamic Phosphorylation of the C Terminus of Hsp70 Regulates the Mitochondrial Import of SOD2 and Redox Balance.
Application : WB
Reactivity : Human
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Package List Price ($)
$ 399