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AKT1 + AKT3 antibody [AT6D5]

Anti-AKT1 + AKT3 antibody [AT6D5] used in Western Blot (WB). GTX57714
Anti-AKT1 + AKT3 antibody [AT6D5] used in Flow cytometry (FACS). GTX57714
Anti-AKT1 + AKT3 antibody [AT6D5] used in Western Blot (WB). GTX57714
Anti-AKT1 + AKT3 antibody [AT6D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57714

Cat. No. GTX57714

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT6D5

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all AKT1 + AKT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Product Note

Based on internal testing result, this antibody does not cross react with human AKT2.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT6D5 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AKT3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2

Cellular Localization

Cytoplasm , Nucleus , Cell membrane , Membrane

Database

DATA IMAGES

Anti-AKT1 + AKT3 antibody [AT6D5] used in Western Blot (WB). GTX57714

GTX57714 WB Image

WB analysis of NIH3T3 whole cell lysate using GTX57714 AKT1 + AKT3 antibody [AT6D5].
Loading : 40 μg
Dilution : 1:1000

Anti-AKT1 + AKT3 antibody [AT6D5] used in Flow cytometry (FACS). GTX57714

GTX57714 FACS Image

FACS analysis of A549 cells using GTX57714 AKT1 + AKT3 antibody [AT6D5].
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

Anti-AKT1 + AKT3 antibody [AT6D5] used in Western Blot (WB). GTX57714

GTX57714 WB Image

WB analysis of various samples using GTX57714 AKT1 + AKT3 antibody [AT6D5].
Lane 1 : Recombinant Human AKT1 protein
Lane 2 : Recombinant Human AKT2 protein
Lane 3 : Recombinant Human AKT3 protein
Loading : 50 ng
Dilution : 1:1000

Anti-AKT1 + AKT3 antibody [AT6D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57714

GTX57714 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57714 AKT1 + AKT3 antibody [AT6D5].
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399