AKT1 antibody [HL1142]

Cat. No. GTX636413

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1142
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Rat, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all AKT1 products

Datasheet

View product citations for antibody GTX636413 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Mouse, Zebrafish, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Database

Gene ID: 207 AKT1
UniProt: P31749 AKT1

Research Area

DATA IMAGES

GTX636413 WB Image

Wild-type (WT) and AKT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636413 IHC-P Image

AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636413 IHC-P Image

AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636413 ICC/IF Image

AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636413 IHC-P Image

AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636413 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 | 10 | 5 | 50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636413 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636413 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

AKT1 antibody [HL1142]

Cat. No. GTX636413

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX636413 WB Image

GTX636413 IHC-P Image

GTX636413 IHC-P Image

GTX636413 ICC/IF Image

GTX636413 IHC-P Image

GTX636413 WB Image

GTX636413 WB Image

GTX636413 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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