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AKT1 antibody [HL1145]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in IHC (Paraffin sections) (IHC-P). GTX636416

Cat. No. GTX636416

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1145

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($419),
25 μl ($169)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Observed MW

60 kDa.

Product Note

This antibody is specific for AKT1 protein and does not cross react with AKT2 or AKT3 protein.

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909979

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

KO/KD

Wild-type (WT) and AKT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in IHC (Paraffin sections) (IHC-P). GTX636416

GTX636416 IHC-P Image

AKT1 antibody [HL1145] detects AKT1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
AKT1 stained by AKT1 antibody [HL1145] (GTX636416) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Fu Y et al. Oncogenesis 2023; 12 (1) : 1 AKT1 regulates UHRF1 protein stability and promotes the resistance to abiraterone in prostate cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 419
$ 169
Custom Conjugations