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AKT1 antibody [HL1145]

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416
Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

Cat. No. GTX636416

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1145

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Product Note

This antibody is specific for AKT1 protein and does not cross react with AKT2 or AKT3 protein.

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909979

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Wild-type (WT) and AKT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:500. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT1 antibody [HL1145] used in Western Blot (WB). GTX636416

GTX636416 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1145] (GTX636416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for AKT1 antibody [HL1145] (GTX636416). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AKT1 antibody [HL1145] (GTX636416). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations