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AKT1 antibody [PKB-175]

Anti-AKT1 antibody [PKB-175] used in Immunoprecipitation (IP). GTX10438
Anti-AKT1 antibody [PKB-175] used in Western Blot (WB). GTX10438

Cat No. GTX10438

Host Mouse
Clonality Monoclonal
Clone Name PKB-175
Isotype IgG1
Application WB, IP, ELISA
Reactivity Human, Mouse, Rat, Bovine, Chicken
Package
50 μl ($289)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5,000
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

MCF7
PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The Akt family of serine/threonine protein kinases (also called protein kinase B/PKB) plays a key role in insulin signaling, cellular survival, and transformation. Akt1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.

Database

Research Area

DATA IMAGES
Anti-AKT1 antibody [PKB-175] used in Immunoprecipitation (IP). GTX10438

GTX10438 IP Image

IP analysis of MCF-7 cells using GTX10438 AKT1 antibody [PKB-175].
Lane 1 : IP from MCF-7 cells using10 μL antibody
Lane 2 : IP from MCF-7 cells using10 μL nonrelevant antibody
Lane 3 : MCF-7 whole cell lysate

Anti-AKT1 antibody [PKB-175] used in Western Blot (WB). GTX10438

GTX10438 WB Image

WB analysis of (1)HeLa (2) HepG2 (3) A549 (4) HEK-293 (5) JURKAT (6) MCF-7 cells using GTX10438 AKT1 antibody [PKB-175] at 1:5,000.

REFERENCE
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REVIEW
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Package List Price ($)
$ 289