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AKT1 antibody

Anti-AKT1 antibody used in Western Blot (WB). GTX110613
Anti-AKT1 antibody used in Immunoprecipitation (IP). GTX110613
Anti-AKT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110613
Anti-AKT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110613
Anti-AKT1 antibody used in Western Blot (WB). GTX110613

Cat No. GTX110613

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HA-AKT transfected HeLa cell , mouse liver

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377404)

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Akt1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Akt Serine/Threonine Kinase 1,Akt,Cws6,Pkb,Pkb-Alpha,Prkba,Rac,Rac-Alpha,Akt1

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-AKT1 antibody used in Western Blot (WB). GTX110613

GTX110613 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX110613 diluted at 1:1000

Anti-AKT1 antibody used in Immunoprecipitation (IP). GTX110613

GTX110613 IP Image

Akt1 antibody immunoprecipitates Akt1 protein in IP experiments.
IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells.
A. 30 μg whole cell extract of Akt1-protein expressing 293T cell
B. Control with 3 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613)
10 % SDS-PAGE
The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-AKT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110613

GTX110613 ICC/IF Image

Akt1 antibody detects Akt1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Akt1 protein stained by Akt1 antibody (GTX110613) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-AKT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110613

GTX110613 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1(GTX110613) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AKT1 antibody used in Western Blot (WB). GTX110613

GTX110613 WB Image

Sample (20 ug of whole cell lysate)
A: HeLa cell lysate
B: HeLa cell transfect with AKT
7.5% SDS PAGE
GTX110613 diluted at 1:10000

REFERENCE
REVIEW

AKT1 antibody Cat No. GTX110613

Rating ( Average 5 based on 1 users reviews)
Application
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169