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AKT1 (phospho Ser124) antibody [GT1268]

Recombinant antibody Orthogonal
Anti-AKT1 (phospho Ser124) antibody [GT1268] used in Western Blot (WB). GTX03180

Cat. No. GTX03180

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1268

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($419)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Product Note

Based on internal testing result, this antibody does not cross with AKT2 and AKT3 proteins.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S124 of human AKT1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-AKT1 (phospho Ser124) antibody [GT1268] used in Western Blot (WB). GTX03180

GTX03180 WB Image

Orthogonal

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser124) antibody [GT1268] (GTX03180) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 419