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AKT2 antibody [1B6]

Anti-AKT2 antibody [1B6] used in Western Blot (WB). GTX83257
Anti-AKT2 antibody [1B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83257
Anti-AKT2 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX83257

Cat. No. GTX83257

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B6

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Rat, Monkey
Package
100 μl ($399)
See all AKT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human AKT2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 2 , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AKT2 antibody [1B6] used in Western Blot (WB). GTX83257

GTX83257 WB Image

WB analysis of A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate using GTX83257 AKT2 antibody [1B6].

Anti-AKT2 antibody [1B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83257

GTX83257 ICC/IF Image

ICC/IF analysis of PANC-1 (left) and HeLa (right) cells using GTX83257 AKT2 antibody [1B6].
Green : AKT2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-AKT2 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX83257

GTX83257 IHC-P Image

IHC-P analysis of human Thymus tissue using GTX83257 AKT2 antibody [1B6].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399